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Vittorio Saggiomo
Приєднався 28 бер 2009
Scientist and Academic (Assistant Professor in BioNanoTechnology, Wageningen, NL)
Microfabrication and microfluidics; 3D Printing for Molecular Life Science; Sensors and Devices;
Human, Chemist, Geek, Gamer, Ukulelist
Twitter @V_Saggiomo
Microfabrication and microfluidics; 3D Printing for Molecular Life Science; Sensors and Devices;
Human, Chemist, Geek, Gamer, Ukulelist
Twitter @V_Saggiomo
ESPressoscope - a DIY WiFi microscope as small as an espresso cup
This is the second version of the “matchboscope”
www.printables.com/model/412753-esp32-microscope-matchboxscope
ua-cam.com/video/iw_hoDRlnpg/v-deo.html
Here we introduce the ESPressoscope, a #microscope as big as an espresso cup :)
We moved away from the ESP32-Cam and are using the #seeedstudio #xiao #ESP32S3 Sense board. It is slightly more expensive (€16-18) but is way more reliable than the previous board.
It can be used to find #tardigrade around your garden and is good enough to see #zooplankton and other microanimals.
The STL, instructions and the bill of materials are in the repository on Printables: www.printables.com/model/585025
This video shows the building process.
For more info, the GitHub, and the work in progress, please check: matchboxscope.github.io
project based on research from me (@V_saggiomo) and Benedict Diederich (@beniroquai)
www.printables.com/model/412753-esp32-microscope-matchboxscope
ua-cam.com/video/iw_hoDRlnpg/v-deo.html
Here we introduce the ESPressoscope, a #microscope as big as an espresso cup :)
We moved away from the ESP32-Cam and are using the #seeedstudio #xiao #ESP32S3 Sense board. It is slightly more expensive (€16-18) but is way more reliable than the previous board.
It can be used to find #tardigrade around your garden and is good enough to see #zooplankton and other microanimals.
The STL, instructions and the bill of materials are in the repository on Printables: www.printables.com/model/585025
This video shows the building process.
For more info, the GitHub, and the work in progress, please check: matchboxscope.github.io
project based on research from me (@V_saggiomo) and Benedict Diederich (@beniroquai)
Переглядів: 1 884
Відео
ESP32 Microscope Assembly Video Instruction
Переглядів 2 тис.Рік тому
Building guide for the ESP32 Microscope. In real-time, so quite slow :D more info: github.com/Matchboxscope/Matchboxscope/blob/master/Matchboxscope.md and here: www.printables.com/model/412753-esp32-microscope
ESP32 Microscope v1 screen recording
Переглядів 1,4 тис.Рік тому
more info and instructions here: github.com/Matchboxscope/Matchboxscope/blob/master/Matchboxscope.md
Capturing pollen on a bike (3D printed particle accelerator)
Переглядів 8592 роки тому
A small 3D printed device for capturing pollen (and other airborne particles) while biking. It is a "particle accelerator" or "single-stage impactor", it is practically a funnel with a slit at the end, accelerating and focussing the airborne particles on a microscopy glass slide. Just by biking around, you'll be able to collect plenty of particles for hours of fun at the microscopy. How many di...
Electron Microscopy Coloring Book
Переглядів 9982 роки тому
SEM (Scanning Electron Microscope) pictures are beautiful and detailed surfaces reconstructions of the sample in greyscale. They are perfect for being colored in post-processing. This is usually done digitally by scientists: to highlight some parts of the image, to enhance contrast, or, in general, to make a pleasant picture. Now, you also have the possibility of doing the coloring by hand, usi...
(closed) Ender 3 for Science (Contest)
Переглядів 1,2 тис.2 роки тому
/update: we have rewarder all the three finalist with a 3D printer Transform your Ender 3 in a scientific instrument.... and win a 3D printer We (me and Emmanuel Reynaud) selected three finalists and now it's your turn to vote which one you like the most. Comment to cast your vote: 1 - Ender Blotting 2 - Ender Electrospin 3 - Ender Automatic Colorimetric Voting will close on the 5th of December...
[closed] Win an Ender 3 (for scientific purposes)
Переглядів 3622 роки тому
Win an Ender 3 and then destroy it for using it as scientific instrument :D We did it recently, and transformed an Ender 3 in a set of 3 (or 4) syringe pumps: ua-cam.com/video/etUp4s1Bq-I/v-deo.html www.sciencedirect.com/science/article/pii/S2468067221000481 github.com/Vsaggiomo/Ender3-syringe-pumps but that's based on the research we do in our lab. What if you need something else? A 3D printer...
The Smallest and Cutest resin 3D printer (Lite3DP)
Переглядів 9 тис.2 роки тому
Possibly the smallest and cutest resin 3D printer (mSLA) in 2021. I can easily print only few mL of resin, making it perfect for developing your own resin without the need of preparing (and wasting) large amount of resin. It's open source, it's based on arduino, 405nm LED, and LCD mask of 320x240, it doesn't need a slicer, and it's modular. It's the Lite3DP! What do you want more? for other 3D ...
Ultra slow motion test Sony Rx100 Va 1000 fps
Переглядів 1,4 тис.3 роки тому
"Fast" (pun intended) test of the ultra slow motion capability of the Sony Rx100 Va. Both outdoor and indoor.
From Ender 3 to DIY Syringe Pumps (Microfluidics capable)
Переглядів 12 тис.3 роки тому
How to transform an Ender 3 in a set of open, easily programmable syringe pumps set. Syringe pumps can be quite expensive, and with limited possibility of automation. For solving this problem, we bough a cheap Ender 3 and repurpose it in a set of 3 syringe pumps. Almost everything needed for the build is in the box, even all the screws and the tools you need for assembly the syringe pumps. The ...
Coronavirus test in a Coffee capsule (CoronaEspresso)
Переглядів 5 тис.3 роки тому
Short lecture on the development of a novel LAMP device which is cheap, reusable, and can be produced in large amounts in a short period of time. The device was designed such not to require chemical exothermic reactions, have limited waste produced and with a minimum cost of the device as a whole. The device was tested for the detection of SARS-CoV2 RNA. We obviously called it CoronaEspresso :D...
The Other Research Methods; a Podcast - Focus Ep2
Переглядів 1683 роки тому
The other research method - a podcast Episode 2 - Focus Today we discuss a little bit about focus: How to focus on writing? Tips and tricks on how to improve your writing and your focus. Guest of today is Tessa Louwerens, a science writer for the Resource. This podcast is part of the course "Research Methods in BioNanoTechnology" Music New Beautiful Day by WinnieTheMoog Link: filmmusic.io/song/...
The Other Research Methods; a Podcast - Creativity Ep1
Переглядів 4543 роки тому
The other research method - a podcast Episode 1 - Creativity Today we discuss a little bit about creativity: what is creativity? why we need creativity in science? How to get creative? We will also talk with Banu from banu.design about creativity, art, and science. This podcast is part of the course "Research Methods in BioNanoTechnology" Music New Beautiful Day by WinnieTheMoog Link: filmmusic...
3D printed PDMS microfluidics tips and tricks (ESCARGOT)
Переглядів 19 тис.3 роки тому
Few years ago we published a method on how to make complex, 3D microfluidic devices in an easy way and how to incorporate external components in the microfluidic device. Years later I still get emails asking for more explanations about the method, and this video should help with some of the questions :) The method is called ESCARGOT (Embedded SCAffold RemovinG Open Technology) onlinelibrary.wil...
3D Printing thousand years old designs (3d printing free model)
Переглядів 2353 роки тому
3D Printing thousand years old designs (3d printing free model)
An iPhone (X, 11, 12) 3D Scanner App that Works? (EM3D: Ethan Makes 3D Scanner)
Переглядів 46 тис.3 роки тому
An iPhone (X, 11, 12) 3D Scanner App that Works? (EM3D: Ethan Makes 3D Scanner)
Cheap DIY Polarising Filter for Microscopy (SW380T polarizing filter?)
Переглядів 4,2 тис.3 роки тому
Cheap DIY Polarising Filter for Microscopy (SW380T polarizing filter?)
3D Printing Linocut? (Printmaking using only a 3D Printer)
Переглядів 3,8 тис.3 роки тому
3D Printing Linocut? (Printmaking using only a 3D Printer)
Swift SW380T (Review) + Raspberry pi = digital microscope
Переглядів 33 тис.3 роки тому
Swift SW380T (Review) Raspberry pi = digital microscope
3D Printed Microscope: Hunting for Tardigrades
Переглядів 12 тис.3 роки тому
3D Printed Microscope: Hunting for Tardigrades
3D printing time lapse ( Octoprint / Octolapse / Raspberry pi 4 HQ camera)
Переглядів 3,7 тис.3 роки тому
3D printing time lapse ( Octoprint / Octolapse / Raspberry pi 4 HQ camera)
Microplastics from 3D printing (Fluorescence Microscopy detection of Fluo Filament Microparticles)
Переглядів 1,6 тис.3 роки тому
Microplastics from 3D printing (Fluorescence Microscopy detection of Fluo Filament Microparticles)
Microplastics from 3D Printing (how to detect them and risk management)
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Microplastics from 3D Printing (how to detect them and risk management)
Microscopy Time lapse (SWIFT SW380T - Raspberry pi 4 HQ Camera)
Переглядів 6 тис.3 роки тому
Microscopy Time lapse (SWIFT SW380T - Raspberry pi 4 HQ Camera)
3D Design/Printing "furry" Coronavirus (SARS-CoV-2, covid19) Spike Protein
Переглядів 4523 роки тому
3D Design/Printing "furry" Coronavirus (SARS-CoV-2, covid19) Spike Protein
Invited lectures Rolf Hut, Neeti Kalyani (Sensors and Devices 06_2)
Переглядів 3013 роки тому
Invited lectures Rolf Hut, Neeti Kalyani (Sensors and Devices 06_2)
Invited lectures 1 Brian Pauw, Gina Ross (Sensors and Devices 06_1 )
Переглядів 6883 роки тому
Invited lectures 1 Brian Pauw, Gina Ross (Sensors and Devices 06_1 )
3D Design and Print your Own Cuvette Holder - 3D Printing in the lab 01
Переглядів 3913 роки тому
3D Design and Print your Own Cuvette Holder - 3D Printing in the lab 01
Traditional Neapolitan "Day of the Dead" Dessert (Nutella + Chocolate + Nuts)
Переглядів 2543 роки тому
Traditional Neapolitan "Day of the Dead" Dessert (Nutella Chocolate Nuts)
Sir .. Instead of mb can I use FTDI to connect ESP32-CAM??
Sir where can i find the code to upload on esp32 cam And after uploading it ,I can connect it to a powerbank right? Can u please explain the procedures after that which involves how to connect it with a phone or a device...and which software is used to stream ...like that!?
here: matchboxscope.github.io/docs/Software/Firmware
Sir can u please tell where is the stl files for 3D printing
literally in the description: www.printables.com/model/412753-esp32-microscope
@@v_saggiomo thanku❤️
Is it possible to estimate the focus distance from the sensor on your project?
that dipends on the distance from the lens to the sensor: matchboxscope.github.io/docs/Introduction
Hey! this is amazing! Can you make a video about the setup (microscope, raspbery pi, screen) .. I'm a noob in raspbery pi
are you still here ? i need help with the rasberry pi stuff like what i need to buy to use the camera like what lens
stil here, but a comment is not enough to explain how a raspberry pi works :D. Any camera module (without any lens) will work
Please,tell me where can i get 60x, i cant find
aliexpress
I love that you could make a design that others could preproduce themselves so easily if they had access to a printer!!
Hello from India. Firstly, a great video... and such amazing creativity... wow! I'm a doctor, and I'm also associated with an NGO here. Was planning on donating an infusion pump for a long time now... but it was too costly. If anyone is interested in selling one of these to me, I'd love to connect with them to discuss further details. Thanks. 👍🏻😊
It looks soo dope
TATTO Magnetic Nano Particle are very harmful in case a person need MRI Scanning. it may lead to Burning during MRI. Anyway I've just wanted to thank you for BioNano Technology course series. they are super interesting and you are amazing teacher/professor 🙏🙏🙏
Hey, recently I tried to build the pumps using the dual+50ml adapters, but the holes for the rod are not aligned, is there any fix exept making holes in the 3d printed parts?
it'a a standard Ender 3? we (and other groups have rebuild it without any problem), are you sure you are using the right axis? can you drop me an email with some pictures?
@@v_saggiomo there is no problem with bought parts, I sent you an email with photos of the problem.
what would happen if you would place something under the microscope that doesnt let light through, like a shiver of rock, crystal, or a butterfly wing and illuminate it from above. Even though these tiny crawling and swimming microorganisms are fascinating from a scientific point of view, me as an artist, want to create beauty; stunning pictures that i can use as they are, or use them as part of a collage, for example. Colour and sharpness of the images is now of the essence....would this microscope be useful in this endeavour?
Good❤❤❤ very good❤❤❤
A bouquet of beautiful red roses dedicated to your beautiful art!!!🌹🌹🌹🌹🌹🌹🌹🌹🌹🌹🌹🌹🌹🌹 Thank you my dear friend from afar!!🌹🌹🌹🌹🌹🌹🌹🌹
49👍👌👌❤❤❤
Good video! No one in my lab did something like this before, but they've put new project in line which includes different micro fluid channels, now gonna try to make them, thank you!
There is an additional benefit not mentioned. If the printing block wears out, you can just print another one, identical! Also to smooth out the print you could put the printing block on your heated bed with something flat and heavy, just briefly, to slightly melt out the imperfections.
I have 3 printers operating in the 3x3 m room, and there is also a Xiaomi air purifier with presumably a HEPA filter. While the air purifier is measuring PM 2.5 at 0-1 which is like the purest you can get, going inside the room after 2-3 hours of simultaneous printing gives me a bad cough right away. IDK, maybe it's the smaller particles like PM 1.0, or the really dry air (28-30% relative humidity) or the oxygen burned out by the high temps, but it's not as harmless and normal as I hoped it would be, additional ventilation is a must. P.S. I'm printing with PLA
Questipn: why raspberry pi and not a normal camera made by swift?
versatility of not having a computer nearby :) (and probably cheaper?)
Music not exactly very relaxing and far too loud. Otherwise, very interesting.
Are you able to view video on a screen as you work the microscope? I just bought the sw380t and don't have a Pi, would you be able to link how to use or set it up? I've seen links for the camera module, I just don't know which pi and how to use it.
Por qué en 40X no logro ver nada? 😅
no, the 40x is not an immersion objective. Do you see black? try to open the diaphragm or increase the light
@@v_saggiomo ahora si pude ver con aceite de inmersión pero es muy cerca de el lente, sabe cómo ajustar el X100?
the X100 is an oil objective and there you need to use oil@@aetherdaniela
it is difficult to use, you need to be very close to the sample, and make very small adjustment. easier to check some video: ua-cam.com/video/baFTRZN_fQU/v-deo.html @@aetherdaniela
Pra el 40X le pones aceite de inmersión?
I'm considering buying this microscope. How are you liking it?
for the price, it's a very good microscope
Can the rasberry pi shoot Raw images or just jpeg? Any information is appreciated. Thank you
I need help, the scan I made is of good quality but when I export it in obj, stl whatever it is the quality deteriorates
i dont know if the paid version is any better but the free one is useless it does loose track all the time impossible to get a scan
are you planning on getting and reviewing the newest version of this printer
yes, at least that's the plan :)
What a beautiful project. Congratulations and thanks for sharing.
Dear Vittorio, I really like your work... However, I am having a problem with the assembly of the camera. I have already ruinned 3 camera modules, and I would like to have more information about how do it properly.
Which problem? Removing the lens? that's just slow and steady.
😚 *Promo sm*
This makes a bit more sense considering the “Face ID” dealio.
Microscopy has gained a hilarious potential risk factor with this creation; accidentally trying to take a sip from my microscope.
i love everything about this! When I found it it fitted my mood so perfectly with the music and the topic
2023 - do you still recommend? I plan on scanning my sculptures
app-wise yes. otherwise there are some handheld scanners available now (for example the revopoint ones)
thank you@@v_saggiomo
Would this work on an ender 5?
well, yes in general terms (programming, motor steps and so on), not sure if the 3D printed adapted parts are the same
Just stumbled onto this channel and had a quick question. Why did you choose ABS and not a water soluble polymer like PVA? is PDMS compatible with H2O? i assume so right? Also greeting from the netherlands!
you're right. It is possible to use PVA and someone did it years after we used ABS/acetone. In 2015, when we did it, PVA was not yet that much available, and very difficult to print. Nowadays it's better. a second point is that ABS/Acetone is faster than PVA/water as acetone is swelling the PDMS just a little bit that favours the dissolution of the ABS. cheers :)
This is very impressive well done!
thanks a lot :)
I am curious, can this be used to view bacterial and fungal spores
you reach 5um, so doubt
I love this! Welldone always sir
Alas, I have seen two resin 3D printers that are much smaller. One was a tiny heirloom printer made of wood and brass where possible. The other was itself mostly 3D printed and fit inside a large Christmas Ornament. That one definitely worked, and printed smaller ornaments.
How much did this cost
around 100euro
Hi thank you for sharing your great technique for that could please share what type of PDMS (I mean viscosity level) and where can I buy it in Europe? Thanks
sylgard 184, VWR or Fernell
This is an awesome project. I've built two but I'm having Wi-Fi connectivity issues with the ESP-32 board, it keeps disconnecting after a while (maybe 5 minutes, I didn't time it). I did some online research and found that this is a common issue with this particular board. Do you have any workaround for this issue?
unfortunately those bords are picky sometimes. try to decrease the resolution and quality, from time to time they reset. We are trying another board, the XIAO SENSE, that seems more reliable, for a little bit more money: matchboxscope.github.io/docs/Hardware/SeeedXiao it's still under development, but, so far it seems to work way better than the standard ESP32
@@v_saggiomo Thank you for the reply! I'll check this board. This is an amazing project, I hope it gets more recognition from the scientific community.
Thank you so much.
Does anyone have a tutorial on how to attach one of these cameras to the microscope?
This is amazing 🤩
I really don't understand. Did you really waste the whole 3D printer to make a syringe pump?
yup, a single syringe pump costs 400€, this 3d printer costs 150€ and you can make 4 syringe pumps...
@@v_saggiomo Thank you for your kind reply. I've got your point but still you don't need to waste the printer. All you need a NEMA 17 step motor, 2 steel rod, 2 lmek 08 uu linear bearing, 2 8mm bearing, a motor coupler, a lead screw with its nut and the arduino. The other parts could be printed. All of them takes 28.27 € acoording to prices in Turkey. I tried it and it works fine. By the way 400 € looks like a good price :) I can deliver hundreds of them for 200€ with a high quality metal parts and touch screen :)
yes, of course you can, but that's not the point. The point is that in a single box you have everything for making them
Very creative got your idea working using off the shelf parts.